// Interfacial Protein Engineering

Advanced
Ice Control.

Ice-binding proteins (IBPs) are interfacial biomolecules that adsorb to specific crystallographic planes of ice, changing ice growth, morphology, and recrystallisation behaviour. We engineer and formulate IBP systems for real-world thermal and cold-chain challenges.

They don’t “stop freezing” — they change how ice behaves at the interface.
Status: Pilot-Ready Formulations for rail assets • Seeking test partners • UK deployment focus
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The Platform

A repeatable system for engineering ice-control at the molecular interface.
01 // Discovery

Identification

Identification and characterisation of ice-binding motifs across taxa (plants, microbes, fish, insects), informed by sequence/structure data and ice-growth assays.

02 // Engineering

Optimisation

Optimisation of binding-surface geometry, environmental stability (pH, salts, drying), and manufacturability — while preserving ice-plane affinity and functional activity.

03 // Deployment

Integration

Integration into coatings and formulations so ice-binding activity is retained where it matters: the ice–surface (or ice–solution) interface under real operating conditions.

Engineering the Interface

We translate biological ice-control into measurable interfacial phenomena: thermal hysteresis (TH), ice recrystallisation inhibition (IRI), dynamic ice shaping, and reduced ice adhesion.
MECHANISM

Adsorption–Inhibition

IBP Ventures develops systems that act by adsorption to ice surfaces, where binding to specific planes perturbs step growth and recrystallisation kinetics. Depending on the IBP type and conditions, this produces measurable IRI and TH — properties widely studied in biology, food science, and cryobiology.

PRECISION

Structure-Function

Many IBPs exhibit plane-selective binding (e.g., basal vs prism/pyramidal faces), consistent with a structured ice-binding surface that matches features of the ice lattice. Structure–function studies show that subtle surface geometry and residue patterning strongly affect binding activity.

IBPs 101

What they do (and don’t do)

IBPs are not bulk antifreeze agents: they generally do not prevent water from freezing under equilibrium conditions. Their value is interfacial control — binding to ice surfaces to modify growth and recrystallisation, which can reduce damage in frozen systems and improve texture stability.

HERITAGE

York & UK Roots

The UK has a strong history in ice-structuring research, including University of York work on plant-derived antifreeze proteins and ice recrystallisation inhibition (e.g., Smallwood et al., carrot AFP) and related "ice structuring protein" terminology.

Sector Solutions

MEDICINE

mRNA & LNP Stability

mRNA–lipid nanoparticles can be sensitive to freezing without appropriate cryoprotectants. Current formulation science relies heavily on sugars and controlled processes. Ice-interface control (including IRI-active materials) is a research direction for reducing freeze-stress in sensitive biologics — requiring careful validation for each formulation.

CRYO-BIOLOGY

IVF & Cryo-Preservation

In reproductive medicine, ice damage is driven by morphology and recrystallisation. IBPs are investigated as additives to suppress recrystallisation and improve post-thaw recovery in sensitive biological materials under defined protocols.

  • Targets ice recrystallisation during freeze–thaw
  • Evaluated case-by-case for biocompatibility
LOGISTICS

Cold Chain Stabilisation

We develop and evaluate ice-interface additives aimed at improving the robustness of temperature-sensitive biologics during freezing, thawing, and temperature excursions — with validation guided by product-specific quality attributes.

SCIENTIFIC REALISM

Validation Metrics

Performance depends on IBP type, concentration, and thermal history. Importantly, IRI does not necessarily correlate with thermal hysteresis, so target metrics must be defined per application (e.g., recrystallisation rate, ice morphology, adhesion force).

Policy Alignment

Our approach aligns with UK priorities around transport resilience, whole-life cost reduction, and environmentally responsible innovation, and is well suited to Innovate UK-style collaborative R&D and rail-sector pilot and deployment pathways.

Selected References (Academic)

  1. Davies, P.L. “Ice-binding proteins: a remarkable diversity of structures for stopping and starting ice growth.” Trends in Biochemical Sciences (2014). https://doi.org/10.1016/j.tibs.2014.09.005
  2. Smallwood, M. et al. “Isolation and characterization of a novel antifreeze protein from carrot (Daucus carota).” Biochemical Journal (1999). https://doi.org/10.1042/bj3400385
  3. Clarke, C.J., Buckley, S.L., Lindner, N. “Ice structuring proteins – a new name for antifreeze proteins.” CryoLetters (2002). PubMed
  4. Buckley, S.L. & Lillford, P.J. “Antifreeze Proteins: Their Structure, Binding and Use.” In: Modern Biopolymer Science, Elsevier (2009). https://doi.org/10.1016/B978-0-12-374195-0.00003-3
  5. Gruneberg, A.K. et al. “Ice recrystallization inhibition activity varies with ice-binding protein type and does not correlate with thermal hysteresis.” Cryobiology (2021). https://doi.org/10.1016/j.cryobiol.2021.01.017
  6. Murray, K.A. & Gibson, M.I. “Chemical approaches to cryopreservation.” Nature Reviews Chemistry (2022). https://doi.org/10.1038/s41570-022-00407-4
  7. Chang, T. & Zhao, G. “Ice Inhibition for Cryopreservation: Materials, Strategies, and Challenges.” Advanced Science (2021). https://doi.org/10.1002/advs.202002425
  8. Kamiya, M. et al. “Stability study of mRNA–lipid nanoparticles exposed to various conditions based on physicochemical properties and protein expression.” Pharmaceutics (2022). https://doi.org/10.3390/pharmaceutics14112357
  9. Kim, B. et al. “Optimization of storage conditions for lipid nanoparticle-formulated self-replicating RNA vaccines.” Journal of Controlled Release (2023). https://doi.org/10.1016/j.jconrel.2022.11.022
  10. Khan, M.D.F.H. et al. “Freeze-Drying of mRNA-LNP Vaccines: A Review.” Vaccines (2025). https://doi.org/10.3390/vaccines13080853
Partnership

Let's Talk Business

We are engaging with investors and strategic partners aligned with infrastructure resilience, climate adaptation, and advanced materials.

IBP VENTURES LTD
REGISTERED NO. 14605472
YORK, UK